Human PIAS3 ELISA Kit

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  • Alternative name

    Human protein inhibitor of activated STAT protein 3 ELISA Kit;Human ZMIZ5 ELISA Kit;Human protein inhibitor of activated STAT 3 ELISA Kit;Human E3 SUMO-protein ligase PIAS3 ELISA Kit;Human zinc finger, MIZ-type containing 5 ELISA Kit;

  • Catalog
    E031556
  • species
    Human
  • GenePIAS3
  • Standard CurveHuman PIAS3 ELISA Kit
  • Other Species Mouse Pias3 ELISA KitRat Pias3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Inhibitor Of Activated STAT 3 (PIAS3). No significant cross-reactivity or interference between Protein Inhibitor Of Activated STAT 3 (PIAS3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman PIAS3 ELISA Kit allows for the in vitro quantitative determination of PIAS3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Inhibitor Of Activated STAT 3 (PIAS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Inhibitor Of Activated STAT 3 (PIAS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human E3 SUMO-protein ligase PIAS3 Protein information
  • Uniprot ID PIAS3_HUMAN
  • Uniprot AC Q9Y6X2; Q9UFI3;
  • UniGene Hs.435761;
  • GeneID 10401
  • KEGG hsa:10401;
  • Human E3 SUMO-protein ligase PIAS3 Protein SEQUENCE
  • SEQUENCE 628 AA; 68017 MW; 7C06EF599D48F87D CRC64;

    MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA

    PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL

    LAPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST

    SSQRFEEAHF TFALTPQQVQ QILTSREVLP GAKCDYTIQV QLRFCLCETS

    CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL

    SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS

    RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD

    AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM

    EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL

    TIESSSDEED LPPTKKHCSV TSAAIPALPG SKGVLTSGHQ PSSVLRSPAM

    GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL

    DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG

    GALREGHGGP LPSGPSLTGC RSDIISLD

  • UCSC uc001eoc.2; human.;


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