Human LTB ELISA Kit

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  • Alternative name

    Human LT-beta ELISA Kit;Human tumor necrosis factor C ELISA Kit;Human TNF-C ELISA Kit;Human tumor necrosis factor ligand superfamily member 3 ELISA Kit;Human TNFC ELISA Kit;Human TNFSF3 ELISA Kit;Human TNLG1C ELISA Kit;Human p33 ELISA Kit;Human lymphotoxin beta ELISA Kit;Human lymphotoxin-beta ELISA Kit;Human TNF superfamily member 3 ELISA Kit;Human lymphotoxin beta (TNF superfamily, member 3) ELISA Kit;Human tumor necrosis factor ligand 1C ELISA Kit;

  • Catalog
    E023173
  • species
    Human
  • GeneLTB
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman LTB ELISA Kit allows for the in vitro quantitative determination of LTB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: LyB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LyB antibody and an LyB-HRP conjugate. The assay sample and buffer are incubated together with LyB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LyB concentration since LyB from samples and LyB-HRP conjugate compete for the anti-LyB antibody binding site. Since the number of sites is limited, as more sites are occupied by LyB from the sample, fewer sites are left to bind LyB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LyB concentration in each sample is interpolated from this standard curve.
  • Human Lymphotoxin-beta Protein information
  • Uniprot ID TNFC_HUMAN
  • Uniprot AC Q06643; P78370; Q52LU8; Q99761;
  • UniGene Hs.376208;
  • GeneID 4050
  • KEGG hsa:4050;
  • Human Lymphotoxin-beta Protein SEQUENCE
  • SEQUENCE 244 AA; 25390 MW; F41569459830ED4C CRC64;

    MGALGLEGRG GRLQGRGSLL LAVAGATSLV TLLLAVPITV LAVLALVPQD

    QGGLVTETAD PGAQAQQGLG FQKLPEEEPE TDLSPGLPAA HLIGAPLKGQ

    GLGWETTKEQ AFLTSGTQFS DAEGLALPQD GLYYLYCLVG YRGRAPPGGG

    DPQGRSVTLR SSLYRAGGAY GPGTPELLLE GAETVTPVLD PARRQGYGPL

    WYTSVGFGGL VQLRRGERVY VNISHPDMVD FARGKTFFGA VMVG

  • UCSC uc003nul.4; human. [Q06643-1];



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