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Human Hs CRP ELISA Kit

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  • Alternative name

    C-reactive protein ELISA KIT; CRP ELISA KIT; PTX1 ELISA KIT
  • Catalog
    E018651
  • species
    Human
  • GeneHs CRP
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of hs-CRP. No significant cross-reactivity or interference between hs-CRP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between hs-CRP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman Hs CRP ELISA Kit allows for the in vitro quantitative determination of Hs CRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the assay: hs-CRP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for hs-CRP. Standards or samples are then added to the microtiter plate wells and hs-CRP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of hs-CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for hs-CRP are added to each well to "sandwich" the hs-CRP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hs-CRP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The hs-CRP concentration in each sample is interpolated from this standard curve.



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