Human BECN1 ELISA Kit

Alternative name
Human coiled-coil myosin-like BCL2-interacting protein ELISA Kit;Human Protein GT197 ELISA Kit;Human GT197 ELISA Kit;Human ATG6 ELISA Kit;Human VPS30 ELISA Kit;Human beclin1 ELISA Kit;Human beclin 1 ELISA Kit;Human beclin-1 ELISA Kit;Human ATG6 autophagy related 6 homolog ELISA Kit;Human beclin 1 (coiled-coil, moesin-like BCL2-interacting protein) ELISA Kit;Human beclin 1, autophagy related ELISA Kit;Human testis secretory sperm-binding protein Li 215e ELISA Kit;
Catalog
E004596
species
Human
GeneBECN1
Standard Curve
Other Species Mouse Becn1 ELISA Kit Bovine BECN1 ELISA Kit Chicken BECN1 ELISA Kit Porcine BECN1 ELISA Kit Rat Becn1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Beclin 1 (BECN1). No significant cross-reactivity or interference between Beclin 1 (BECN1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.063ng/mL.
Intended UseHuman BECN1 ELISA Kit allows for the in vitro quantitative determination of BECN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beclin 1 (BECN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beclin 1 (BECN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beclin 1 (BECN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beclin 1 (BECN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Beclin-1-C 37 kDa Protein information
Uniprot ID BECN1_HUMAN
Uniprot AC Q14457; B2R6N7; O75595; Q9UNA8;
UniGene Hs.716464;
GeneID 8678
KEGG hsa:8678;
Human Beclin-1-C 37 kDa Protein SEQUENCE
SEQUENCE 450 AA; 51896 MW; ABF0C2DD7087473C CRC64;
MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA
QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI
GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT
QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED
VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV
ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW
NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP
LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK
GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK
UCSC uc002ibn.3; human.;

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Human BECN1 ELISA Kit