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Human ATXN3 ELISA Kit

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  • Alternative name

    Human Machado-Joseph disease protein 1 ELISA Kit;Human spinocerebellar ataxia type 3 protein ELISA Kit;Human ATX3 ELISA Kit;Human MJD ELISA Kit;Human MJD1 ELISA Kit;Human SCA3 ELISA Kit;Human AT3 ELISA Kit;Human JOS ELISA Kit;Human ataxin 3 ELISA Kit;Human ataxin-3 ELISA Kit;Human josephin ELISA Kit;Human olivopontocerebellar ataxia 3 ELISA Kit;
  • Catalog
    E003912
  • species
    Human
  • GeneATXN3
  • Standard CurveHuman ATXN3 ELISA Kit
  • Other Species Mouse Atxn3 ELISA KitChicken ATXN3 ELISA KitRat Atxn3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATXN3. No significant cross-reactivity or interference between ATXN3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATXN3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATXN3 ELISA Kit allows for the in vitro quantitative determination of ATXN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical    Principle of the assay: ATXN3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATXN3 antibody and an ATXN3-HRP conjugate. The assay sample and buffer are incubated together with ATXN3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATXN3 concentration since ATXN3 from samples and ATXN3-HRP conjugate compete for the anti-ATXN3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATXN3 from the sample, fewer sites are left to bind ATXN3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATXN3 concentration in each sample is interpolated from this standard curve.
  • Human Ataxin-3 Protein information
  • Uniprot ID ATX3_HUMAN
  • Uniprot AC Q96TC3; Q96TC4; Q9H3N0;
  • UniGene Hs.532632;
  • GeneID 4287
  • KEGG hsa:4287;
  • Human Ataxin-3 Protein SEQUENCE

  • SEQUENCE 364 AA; 41781 MW; 4B2477EB67C30EFF CRC64;

    MESIFHEKQE GSLCAQHCLN NLLQGEYFSP VELSSIAHQL DEEERMRMAE

    GGVTSEDYRT FLQQPSGNMD DSGFFSIQVI SNALKVWGLE LILFNSPEYQ

    RLRIDPINER SFICNYKEHW FTVRKLGKQW FNLNSLLTGP ELISDTYLAL

    FLAQLQQEGY SIFVVKGDLP DCEADQLLQM IRVQQMHRPK LIGEELAQLK

    EQRVHKTDLE RVLEANDGSG MLDEDEEDLQ RALALSRQEI DMEDEEADLR

    RAIQLSMQGS SRNISQDMTQ TSGTNLTSEE LRKRREAYFE KQQQKQQQQQ

    QQQQQGDLSG QSSHPCERPA TSSGALGSDL GKACSPFIMF ATFTLYLTYE

    LHVIFALHYS SFPL

  • UCSC uc001yac.5; human. [P54252-1];


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