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Alternative name
Human high mobility group nucleosome-binding domain-containing protein 1 ELISA Kit;Human HMG14 ELISA Kit;Human high mobility group nucleosome binding domain 1 ELISA Kit;Human non-histone chromosomal protein HMG-14 ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 14 ELISA Kit;Human high-mobility group nucleosome binding 1 ELISA Kit;Human nonhistone chromosomal protein HMG-14 ELISA Kit;
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Catalog
E018620
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species
Human
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GeneHMGN1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1).
No significant cross-reactivity or interference between High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.058ng/mL.
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Intended UseHuman HMGN1 ELISA Kit allows for the in vitro quantitative determination of HMGN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Non-histone chromosomal protein HMG-14 Protein information
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Uniprot ID
HMGN1_HUMAN
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Uniprot AC
P05114; Q3KQR8;
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UniGene
Hs.356285;
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GeneID
3150
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KEGG
hsa:3150;
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Human Non-histone chromosomal protein HMG-14 Protein SEQUENCE
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SEQUENCE 100 AA; 10659 MW; 8F4CB5374D51FBF3 CRC64;
MPKRKVSSAE GAAKEEPKRR SARLSAKPPA KVEAKPKKAA AKDKSSDKKV
QTKGKRGAKG
KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD
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UCSC
uc002yxo.4; human.;
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