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Human C.met ELISA Kit

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  • Alternative name

    Hepatocyte growth factor receptor ELISA KIT; HGF/SF receptor ELISA KIT; Proto-oncogene c-Met ELISA KIT; Scatter factor receptor ELISA KIT; SF receptor ELISA KIT; Tyrosine-protein kinase MetMET ELISA KIT; HGF receptor ELISA KIT; SF receptor ELISA KIT
  • Catalog
    E005701
  • species
    Human
  • GeneC.met
  • Standard CurveHuman C.met ELISA Kit
  • Other Species Human c-MET/HGFR ELISA KitHuman c-MET ELISA KitMouse C-MET ELISA KitMouse C.met ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman C.met ELISA Kit allows for the in vitro quantitative determination of C.met , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical    Principle of the Assay: C.met ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C.met. Standards or samples are then added to the microtiter plate wells and C.met if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C.met present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for C.met are added to each well to "sandwich" the C.met immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C.met and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C.met concentration in each sample is interpolated from this standard curve.


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