Human GTF2F1 ELISA Kit

Alternative name
Human general transcription factor IIF 74 kDa subunit ELISA Kit;Human transcription initiation factor IIF subunit alpha ELISA Kit;Human TFIIF-alpha ELISA Kit;Human transcription initiation factor RAP74 ELISA Kit;Human RAP74 ELISA Kit;Human BTF4 ELISA Kit;Human TF2F1 ELISA Kit;Human TFIIF ELISA Kit;Human general transcription factor IIF subunit 1 ELISA Kit;Human general transcription factor IIF, polypeptide 1, 74kDa ELISA Kit;
Catalog
E017680
species
Human
GeneGTF2F1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GTF2F1. No significant cross-reactivity or interference between Human GTF2F1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GTF2F1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman GTF2F1 ELISA Kit allows for the in vitro quantitative determination of GTF2F1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GTF2F1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GTF2F1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GTF2F1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GTF2F1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GTF2F1. You can calculate the concentration of Human GTF2F1 in the samples by comparing the OD of the samples to the standard curve.
Human General transcription factor IIF subunit 1 Protein information
Uniprot ID T2FA_HUMAN
Uniprot AC P35269; B2RCS0; Q9BWN0;
UniGene Hs.68257;
GeneID 2962
KEGG hsa:2962;
Human General transcription factor IIF subunit 1 Protein SEQUENCE
SEQUENCE 517 AA; 58240 MW; 1032B04A36BDC24F CRC64;
MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD
LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL
RVNGKSGRKF KGIKKGGVTE NTSYYIFTQC PDGAFEAFPV HNWYNFTPLA
RHRTLTAEEA EEEWERRNKV LNHFSIMQQR RLKDQDQDED EEEKEKRGRR
KASELRIHDL EDDLEMSSDA SDASGEEGGR VPKAKKKAPL AKGGRKKKKK
KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SQEEPESKAK APQQEEGPKG
VDEQSDSSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSEESDSSEE
SDIDSEASSA LFMAKKKTPP KRERKPSGGS SRGNSRPGTP SAEGGSTSST
LRAAASKLEQ GKRVSEMPAA KRLRLDTGPQ SLSGKSTPQP PSGKTTPNSG
DVQVTEDAVR RYLTRKPMTT KDLLKKFQTK KTGLSSEQTV NVLAQILKRL
NPERKMINDK MHFSLKE
UCSC uc002meq.3; human.;

Category

Human GTF2F1 ELISA Kit