Human MME ELISA Kit

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  • Alternative name

    Human atriopeptidase ELISA Kit;Human common acute lymphocytic leukemia antigen ELISA Kit;Human CALLA ELISA Kit;Human Enkephalinase ELISA Kit;Human neutral endopeptidase 24.11 ELISA Kit;Human NEP ELISA Kit;Human Neutral endopeptidase ELISA Kit;Human skin fibroblast elastase ELISA Kit;Human SFE ELISA Kit;Human EPN ELISA Kit;Human CD10 ELISA Kit;Human CMT2T ELISA Kit;Human SCA43 ELISA Kit;Human membrane metalloendopeptidase ELISA Kit;Human neprilysin ELISA Kit;Human membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) ELISA Kit;Human membrane metallo-endopeptidase variant 1 ELISA Kit;Human membrane metallo-endopeptidase variant 2 ELISA Kit;Human neprilysin-390 ELISA Kit;Human neprilysin-411 ELISA Kit;

  • Catalog
    E007260
  • species
    Human
  • GeneMME
  • Other Species Mouse Mme ELISA KitPorcine MME ELISA KitRabbit MME ELISA KitRat Mme ELISA Kit
  • SpecificityNatural and recombinant human CD10
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman MME ELISA Kit allows for the in vitro quantitative determination of MME , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD10 has been precoated onto 96-well plates. Standards(CHO, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD10 amount of sample captured in plate. Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
  • Human Neprilysin Protein information
  • Uniprot ID NEP_HUMAN
  • Uniprot AC TAYVDFMI SVARLIRQEE RLPIDENQLA LEMNKVMELE KEIANATAKP EDRNDPMLLY
  • UniGene Hs.307734;
  • GeneID 4311
  • KEGG hsa:4311;
  • Human Neprilysin Protein SEQUENCE
  • SEQUENCE 750 AA; 85514 MW; BCF3827C39898630 CRC64;

    MGKSESQMDI TDINTPKPKK KQRWTPLEIS LSVLVLLLTI IAVTMIALYA

    TYDDGICKSS DCIKSAARLI QNMDATTEPC TDFFKYACGG WLKRNVIPET

    SSRYGNFDIL RDELEVVLKD VLQEPKTEDI VAVQKAKALY RSCINESAID

    SRGGEPLLKL LPDIYGWPVA TENWEQKYGA SWTAEKAIAQ LNSKYGKKVL

    INLFVGTDDK NSVNHVIHID QPRLGLPSRD YYECTGIYKE ACTAYVDFMI

    SVARLIRQEE RLPIDENQLA LEMNKVMELE KEIANATAKP EDRNDPMLLY

    NKMTLAQIQN NFSLEINGKP FSWLNFTNEI MSTVNISITN EEDVVVYAPE

    YLTKLKPILT KYSARDLQNL MSWRFIMDLV SSLSRTYKES RNAFRKALYG

    TTSETATWRR CANYVNGNME NAVGRLYVEA AFAGESKHVV EDLIAQIREV

    FIQTLDDLTW MDAETKKRAE EKALAIKERI GYPDDIVSND NKLNNEYLEL

    NYKEDEYFEN IIQNLKFSQS KQLKKLREKV DKDEWISGAA VVNAFYSSGR

    NQIVFPAGIL QPPFFSAQQS NSLNYGGIGM VIGHEITHGF DDNGRNFNKD

    GDLVDWWTQQ SASNFKEQSQ CMVYQYGNFS WDLAGGQHLN GINTLGENIA

    DNGGLGQAYR AYQNYIKKNG EEKLLPGLDL NHKQLFFLNF AQVWCGTYRP

    EYAVNSIKTD VHSPGNFRII GTLQNSAEFS EAFHCRKNSY MNPEKKCRVW

  • UCSC uc003fab.2; human.;




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