Human LIFR ELISA Kit

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  • Alternative name

    Human LIF receptor ELISA Kit;Human LIF-R ELISA Kit;Human CD118 ELISA Kit;Human SJS2 ELISA Kit;Human STWS ELISA Kit;Human SWS ELISA Kit;Human leukemia inhibitory factor receptor alpha ELISA Kit;Human leukemia inhibitory factor receptor ELISA Kit;Human CD118 antigen ELISA Kit;

  • Catalog
    E022559
  • species
    Human
  • GeneLIFR
  • Standard CurveHuman LIFR ELISA Kit
  • Other Species Mouse Lifr ELISA KitCanine LIFR ELISA KitRat Lifr ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1U/ml.
  • Intended UseHuman LIFR ELISA Kit allows for the in vitro quantitative determination of LIFR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the Assay: LIFR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LIFR. Standards or samples are then added to the microtiter plate wells and LIFR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LIFR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for LIFR are added to each well to "sandwich" the LIFR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LIFR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LIFR concentration in each sample is interpolated from this standard curve.
  • Human Leukemia inhibitory factor receptor Protein information
  • Uniprot ID LIFR_HUMAN
  • Uniprot AC P42702; Q6LCD9;
  • UniGene Hs.133421; Hs.657602;
  • GeneID 3977
  • KEGG hsa:3977;
  • Human Leukemia inhibitory factor receptor Protein SEQUENCE
  • SEQUENCE 1097 AA; 123743 MW; C8602897E359FCE5 CRC64;

    MMDIYVCLKR PSWMVDNKRM RTASNFQWLL STFILLYLMN QVNSQKKGAP

    HDLKCVTNNL QVWNCSWKAP SGTGRGTDYE VCIENRSRSC YQLEKTSIKI

    PALSHGDYEI TINSLHDFGS STSKFTLNEQ NVSLIPDTPE ILNLSADFST

    STLYLKWNDR GSVFPHRSNV IWEIKVLRKE SMELVKLVTH NTTLNGKDTL

    HHWSWASDMP LECAIHFVEI RCYIDNLHFS GLEEWSDWSP VKNISWIPDS

    QTKVFPQDKV ILVGSDITFC CVSQEKVLSA LIGHTNCPLI HLDGENVAIK

    IRNISVSASS GTNVVFTTED NIFGTVIFAG YPPDTPQQLN CETHDLKEII

    CSWNPGRVTA LVGPRATSYT LVESFSGKYV RLKRAEAPTN ESYQLLFQML

    PNQEIYNFTL NAHNPLGRSQ STILVNITEK VYPHTPTSFK VKDINSTAVK

    LSWHLPGNFA KINFLCEIEI KKSNSVQEQR NVTIKGVENS SYLVALDKLN

    PYTLYTFRIR CSTETFWKWS KWSNKKQHLT TEASPSKGPD TWREWSSDGK

    NLIIYWKPLP INEANGKILS YNVSCSSDEE TQSLSEIPDP QHKAEIRLDK

    NDYIISVVAK NSVGSSPPSK IASMEIPNDD LKIEQVVGMG KGILLTWHYD

    PNMTCDYVIK WCNSSRSEPC LMDWRKVPSN STETVIESDE FRPGIRYNFF

    LYGCRNQGYQ LLRSMIGYIE ELAPIVAPNF TVEDTSADSI LVKWEDIPVE

    ELRGFLRGYL FYFGKGERDT SKMRVLESGR SDIKVKNITD ISQKTLRIAD

    LQGKTSYHLV LRAYTDGGVG PEKSMYVVTK ENSVGLIIAI LIPVAVAVIV

    GVVTSILCYR KREWIKETFY PDIPNPENCK ALQFQKSVCE GSSALKTLEM

    NPCTPNNVEV LETRSAFPKI EDTEIISPVA ERPEDRSDAE PENHVVVSYC

    PPIIEEEIPN PAADEAGGTA QVIYIDVQSM YQPQAKPEEE QENDPVGGAG

    YKPQMHLPIN STVEDIAAEE DLDKTAGYRP QANVNTWNLV SPDSPRSIDS

    NSEIVSFGSP CSINSRQFLI PPKDEDSPKS NGGGWSFTNF FQNKPND

  • UCSC uc003jli.3; human. [P42702-1];



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