Human GZMK ELISA Kit

Alternative name
Human fragmentin-3 ELISA Kit;Human Granzyme-3 ELISA Kit;Human NK-tryptase-2 ELISA Kit;Human NK-Tryp-2 ELISA Kit;Human TRYP2 ELISA Kit;Human granzyme K ELISA Kit;Human granzyme 3 ELISA Kit;Human granzyme K (granzyme 3; tryptase II) ELISA Kit;Human granzyme K (serine protease, granzyme 3; tryptase II) ELISA Kit;Human tryptase II ELISA Kit;
Catalog
E017446
species
Human
GeneGZMK
Standard Curve
Other Species Mouse Gzmk ELISA Kit Rat Gzmk ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Granzyme K (GZMK). No significant cross-reactivity or interference between Granzyme K (GZMK) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 12.4pg/mL.
Intended UseHuman GZMK ELISA Kit allows for the in vitro quantitative determination of GZMK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme K (GZMK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme K (GZMK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme K (GZMK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granzyme K (GZMK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Granzyme K Protein information
Uniprot ID GRAK_HUMAN
Uniprot AC P49863; B2R563;
UniGene Hs.277937;
GeneID 3003
KEGG hsa:3003;
Human Granzyme K Protein SEQUENCE
SEQUENCE 264 AA; 28882 MW; 2A93FDBAF9286CC5 CRC64;
MTKFSSFSLF FLIVGAYMTH VCFNMEIIGG KEVSPHSRPF MASIQYGGHH
VCGGVLIDPQ WVLTAAHCQY RFTKGQSPTV VLGAHSLSKN EASKQTLEIK
KFIPFSRVTS DPQSNDIMLV KLQTAAKLNK HVKMLHIRSK TSLRSGTKCK
VTGWGATDPD SLRPSDTLRE VTVTVLSRKL CNSQSYYNGD PFITKDMVCA
GDAKGQKDSC KGDSGGPLIC KGVFHAIVSG GHECGVATKP GIYTLLTKKY
QTWIKSNLVP PHTN
UCSC uc003jpl.2; human.;

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Human GZMK ELISA Kit