Mouse Gaa ELISA Kit

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  • Alternative name

    Mouse acid maltase ELISA Kit;Mouse E430018M07Rik ELISA Kit;Mouse glucosidase, alpha, acid ELISA Kit;Mouse lysosomal alpha-glucosidase ELISA Kit;

  • Catalog
    E054300
  • species
    Mouse
  • GeneGaa
  • Standard CurveMouse Gaa ELISA Kit
  • Other Species Human GAA ELISA KitBovine GAA ELISA KitRat Gaa ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucosidase Alpha, Acid (GaA). No significant cross-reactivity or interference between Glucosidase Alpha, Acid (GaA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 14.3pg/mL.
  • Intended UseMouse Gaa ELISA Kit allows for the in vitro quantitative determination of Gaa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid (GaA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Alpha, Acid (GaA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Alpha, Acid (GaA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucosidase Alpha, Acid (GaA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Lysosomal alpha-glucosidase Protein information
  • Uniprot ID LYAG_MOUSE
  • Uniprot AC P70699; Q3UJB2; Q8BGI6; Q91Z45;
  • UniGene Mm.4793;
  • GeneID 14387
  • KEGG mmu:14387;
  • Mouse Lysosomal alpha-glucosidase Protein SEQUENCE
  • SEQUENCE 953 AA; 106248 MW; 956B89685FB5FF81 CRC64;

    MNIRKPLCSN SVVGACTLIS LTTAVILGHL MLRELMLLPQ DLHESSSGLW

    KTYRPHHQEG YKPGPLHIQE QTEQPKEAPT QCDVPPSSRF DCAPDKGISQ

    EQCEARGCCY VPAGQVLKEP QIGQPWCFFP PSYPSYRLEN LSSTESGYTA

    TLTRTSPTFF PKDVLTLQLE VLMETDSRLH FKIKDPASKR YEVPLETPRV

    LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF FADQFLQLST

    SLPSQHITGL GEHLSPLMLS TDWARITLWN RDTPPSQGTN LYGSHPFYLA

    LEDGGLAHGV FLLNSNAMDV ILQPSPALTW RSTGGILDVY VFLGPEPKSV

    VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV

    QWNDLDYMDA RRDFTFNQDS FADFPDMVRE LHQDGRRYMM IVDPAISSAG

    PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGTTAFPDF TNPETLDWWQ

    DMVSEFHAQV PFDGMWLDMN EPSNFVRGSQ QGCPNNELEN PPYVPGVVGG

    ILQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR

    STFSGHGRYA GHWTGDVRSS WEHLAYSVPD ILQFNLLGVP LVGADICGFI

    GDTSEELCVR WTQLGAFYPF MRNHNDLNSV PQEPYRFSET AQQAMRKAFA

    LRYALLPYLY TLFHRAHVRG DTVARPLFLE FPEDPSTWSV DRQLLWGPAL

    LITPVLEPGK TEVTGYFPKG TWYNMQMVSV DSLGTLPSPS SASSFRSAVQ

    SKGQWLTLEA PLDTINVHLR EGYIIPLQGP SLTTTESRKQ PMALAVALTA

    SGEADGELFW DDGESLAVLE RGAYTLVTFS AKNNTIVNKL VRVTKEGAEL

    QLREVTVLGV ATAPTQVLSN GIPVSNFTYS PDNKSLAIPV SLLMGELFQI

    SWS

  • UCSC uc007mqg.2; mouse.;


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