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Human GYPE ELISA Kit

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  • Alternative name

    Human GPE ELISA Kit;Human MNS ELISA Kit;Human MiIX ELISA Kit;Human glycophorin E (MNS blood group) ELISA Kit;Human glycophorin-E ELISA Kit;
  • Catalog
    E017116
  • species
    Human
  • GeneGYPE
  • Standard CurveHuman GYPE ELISA Kit
  • Other Species Mouse GYPE ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman GYPE ELISA Kit allows for the in vitro quantitative determination of GYPE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This GYPE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GYPE. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: GYPE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GYPE antibody and an GYPE-HRP conjugate. The assay sample and buffer are incubated together with GYPE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GYPE concentration since GYPE from samples and GYPE-HRP conjugate compete for the anti-GYPE antibody binding site. Since the number of sites is limited, as more sites are occupied by GYPE from the sample, fewer sites are left to bind GYPE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GYPE concentration in each sample is interpolated from this standard curve.
  • Human Glycophorin-E Protein information
  • Uniprot ID GLPE_HUMAN
  • Uniprot AC P15421; D3DNZ5;
  • UniGene Hs.654368; Hs.728753;
  • GeneID 2996
  • KEGG hsa:2996;
  • Human Glycophorin-E Protein SEQUENCE

  • SEQUENCE 78 AA; 8463 MW; F1F5B6EE5CFB3EB3 CRC64;

    MYGKIIFVLL LSGIVSISAS STTGVAMHTS TSSSVTKSYI SSQTNGITLI

    NWWAMARVIF EVMLVVVGMI ILISYCIR

  • UCSC uc003ijj.4; human.;



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