-
Alternative name
Lactotransferrin ELISA KIT; Growth-inhibiting protein 12 ELISA KIT; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H ELISA KIT; Lfcin-H ELISA KIT; Kaliocin-1 ELISA KIT; Lactoferroxin-A ELISA KIT; Lactoferroxin-B ELISA KIT; Lactoferroxin-CLTF ELISA KIT; GIG12 ELISA KIT; LF ELISA KIT; Lactoferrin ELISA KIT; Lfcin-H ELISA KIT
-
Catalog
E059005
-
species
Mouse
-
GeneLF/LTF
-
SpecificityThis kit recognizes natural and recombinant Mouse LF/LTF. No significant cross-reactivity or interference between Mouse LF/LTF and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity37.5pg/mL
-
Intended UseMouse LF/LTF ELISA Kit allows for the in vitro quantitative determination of LF/LTF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse LF/LTF concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LF/LTF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LF/LTF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LF/LTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LF/LTF. You can calculate the concentration of LF/LTF in the samples by comparing the OD of the samples to the standard curve.
-