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Alternative name
Basigin ELISA KIT; 5F7 ELISA KIT; Collagenase stimulatory factor ELISA KIT; Extracellular matrix metalloproteinase inducer ELISA KIT; EMMPRIN ELISA KIT; Leukocyte activation antigen M6 ELISA KIT; OK blood group antigen ELISA KIT; Tumor cell-derived collagenase stimulatory factor ELISA KIT; TCSF ELISA KIT; CD_antigen: CD147BSG ELISA KIT; UNQ6505/PRO21383 ELISA KIT; EMMPRIN ELISA KIT; TCSF ELISA KIT
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Catalog
E013889
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species
Human
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GeneCD147/EMMPRIN
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Standard Curve
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman CD147/EMMPRIN ELISA Kit allows for the in vitro quantitative determination of CD147/EMMPRIN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This CD147 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCD147 in the sample, thisCD147 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCD147 concentration. The concentration ofCD147 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This CD147 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCD147. Standards or samples are then added to the microtiter plate wells andCD147 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCD147 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCD147 are added to each well to "sandwich" theCD147 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCD147 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
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