Mouse Grem2 ELISA Kit

Alternative name
Mouse cysteine knot superfamily 1, BMP antagonist 2 ELISA Kit;Mouse protein related to DAN and cerberus ELISA Kit;Mouse Prdc ELISA Kit;Mouse Cktsf1b2 ELISA Kit;Mouse Gremlin2 ELISA Kit;Mouse gremlin 2, DAN family BMP antagonist ELISA Kit;Mouse gremlin-2 ELISA Kit;Mouse Gremlin1 ELISA Kit;Mouse gremlin 2 homolog, cysteine knot superfamily ELISA Kit;
Catalog
E054838
species
Mouse
GeneGrem2
SpecificityNatural and recombinant Mouse GREM2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseMouse Grem2 ELISA Kit allows for the in vitro quantitative determination of Grem2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Mouse GREM2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GREM2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: R22-Q168) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GREM2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse GREM2 amount of sample captured in plate. Background/Introduction: GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.
Mouse Gremlin-2 Protein information
Uniprot ID GREM2_MOUSE
Uniprot AC O88273;
UniGene Mm.25760;
GeneID 23893
KEGG mmu:23893;
Mouse Gremlin-2 Protein SEQUENCE
SEQUENCE 168 AA; 19334 MW; 6361C1581D49C281 CRC64;
MFWKLSLTLL LVAVLVKVAE TRKNRPAGAI PSPYKDGSSN NSERWHHQIK
EVLASSQEAL VVTERKYLKS DWCKTQPLRQ TVSEEGCRSR TILNRFCYGQ
CNSFYIPRHV KKEEDSFQSC AFCKPQRVTS VIVELECPGL DPPFRIKKIQ
KVKHCRCMSV NLSDSDKQ
UCSC uc007dtf.1; mouse.;

Mouse Grem2 ELISA Kit

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