Human gp130 ELISA Kit

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  • Alternative name

    Interleukin-6 receptor subunit beta ELISA KIT; CDw130 ELISA KIT; Interleukin-6 signal transducer ELISA KIT; Membrane glycoprotein 130 ELISA KIT; gp130 ELISA KIT; Oncostatin-M receptor subunit alpha ELISA KIT; CD_antigen: CD130IL6ST ELISA KIT; IL-6 receptor subunit beta ELISA KIT; IL-6R subunit beta ELISA KIT; IL-6R-beta ELISA KIT; IL-6RB ELISA KIT; gp130 ELISA KIT

  • Catalog
    E016576
  • species
    Human
  • Genegp130
  • Standard CurveHuman gp130 ELISA Kit
  • Other Species Human gp130/CD130 ELISA KitHuman Gp130/IL6ST ELISA KitMouse Gp130/IL6ST ELISA KitMouse gp130 ELISA KitMouse gp130/CD130 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman gp130 ELISA Kit allows for the in vitro quantitative determination of gp130 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: gp130 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for gp130. Standards or samples are then added to the microtiter plate wells and gp130 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of gp130 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for gp130 are added to each well to "sandwich" the gp130 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain gp130 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gp130 concentration in each sample is interpolated from this standard curve.



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