Human MIF ELISA Kit

Alternative name
Human MIF ELISA Kit;Human Glycosylation-inhibiting factor ELISA Kit;Human GIF ELISA Kit;Human L-dopachrome isomerase ELISA Kit;Human L-dopachrome tautomerase ELISA Kit;Human phenylpyruvate tautomerase ELISA Kit;Human GLIF ELISA Kit;Human MMIF ELISA Kit;Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) ELISA Kit;Human macrophage migration inhibitory factor ELISA Kit;
Catalog
E023406
species
Human
GeneMIF
SpecificityThis assay recognizes recombinant and natural human MIF. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.02 ng/mL
Intended UseHuman MIF ELISA Kit allows for the in vitro quantitative determination of MIF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses||This immunoassay kit allows for the in vitro quantitative determination of human Macrophage migration inhibitory factor, MIF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MIF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MIF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Macrophage migration inhibitory factor Protein information
Uniprot ID MIF_HUMAN
Uniprot AC P14174; A5Z1R8; B2R4S3; Q2V4Y5; Q6FHV0;
UniGene Hs.407995;
GeneID 4282
KEGG hsa:4282;
Human Macrophage migration inhibitory factor Protein SEQUENCE
SEQUENCE 115 AA; 12476 MW; 56D51107C05286B2 CRC64;
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY
DMNAANVGWN NSTFA
UCSC uc002zyr.2; human.;

Human MIF ELISA Kit

Contact Us