Human RABGGTA ELISA Kit

Alternative name
Human geranylgeranyl transferase type II subunit alpha ELISA Kit;Human rab geranyl-geranyltransferase subunit alpha ELISA Kit;Human Rab GG transferase alpha ELISA Kit;Human Rab GGTase alpha ELISA Kit;Human Rab geranylgeranyltransferase subunit alpha ELISA Kit;Human PTAR3 ELISA Kit;Human Rab geranylgeranyltransferase alpha subunit ELISA Kit;Human geranylgeranyl transferase type-2 subunit alpha ELISA Kit;Human protein prenyltransferase alpha subunit repeat containing 3 ELISA Kit;
Catalog
E016377
species
Human
GeneRABGGTA
SpecificityThis assay has high sensitivity and excellent specificity for detection of RABGGTa. No significant cross-reactivity or interference between RABGGTa and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RABGGTa and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman RABGGTA ELISA Kit allows for the in vitro quantitative determination of RABGGTA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses||This RABGGTa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RABGGTa. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: RABGGTa ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-RABGGTa antibody and an RABGGTa-HRP conjugate. The assay sample and buffer are incubated together with RABGGTa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RABGGTa concentration since RABGGTa from samples and RABGGTa-HRP conjugate compete for the anti-RABGGTa antibody binding site. Since the number of sites is limited, as more sites are occupied by RABGGTa from the sample, fewer sites are left to bind RABGGTa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RABGGTa concentration in each sample is interpolated from this standard curve.
Human Geranylgeranyl transferase type-2 subunit alpha Protein information
Uniprot ID PGTA_HUMAN
Uniprot AC Q92696; A8K5N2; D3DS69;
UniGene Hs.377992;
GeneID 5875
KEGG hsa:5875;
Human Geranylgeranyl transferase type-2 subunit alpha Protein SEQUENCE
SEQUENCE 567 AA; 65072 MW; ABA1AFFC8A496C5F CRC64;
MHGRLKVKTS EEQAEAKRLE REQKLKLYQS ATQAVFQKRQ AGELDESVLE
LTSQILGANP DFATLWNCRR EVLQQLETQK SPEELAALVK AELGFLESCL
RVNPKSYGTW HHRCWLLGRL PEPNWTRELE LCARFLEVDE RNFHCWDYRR
FVATQAAVPP AEELAFTDSL ITRNFSNYSS WHYRSCLLPQ LHPQPDSGPQ
GRLPEDVLLK ELELVQNAFF TDPNDQSAWF YHRWLLGRAD PQDALRCLHV
SRDEACLTVS FSRPLLVGSR MEILLLMVDD SPLIVEWRTP DGRNRPSHVW
LCDLPAASLN DQLPQHTFRV IWTAGDVQKE CVLLKGRQEG WCRDSTTDEQ
LFRCELSVEK STVLQSELES CKELQELEPE NKWCLLTIIL LMRALDPLLY
EKETLQYFQT LKAVDPMRAT YLDDLRSKFL LENSVLKMEY AEVRVLHLAH
KDLTVLCHLE QLLLVTHLDL SHNRLRTLPP ALAALRCLEV LQASDNAIES
LDGVTNLPRL QELLLCNNRL QQPAVLQPLA SCPRLVLLNL QGNPLCQAVG
ILEQLAELLP SVSSVLT
UCSC uc001wof.5; human.;

Category

Human RABGGTA ELISA Kit