Human F3 ELISA Kit

Alternative name
Human TF ELISA Kit;Human Coagulation factor III ELISA Kit;Human Thromboplastin ELISA Kit;Human CD142 ELISA Kit;Human TFA ELISA Kit;Human coagulation factor III, tissue factor ELISA Kit;Human tissue factor ELISA Kit;Human coagulation factor III (thromboplastin, tissue factor) ELISA Kit;
Catalog
E038391
species
Human
GeneF3
SpecificityNatural and recombinant human Tissue factor
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman F3 ELISA Kit allows for the in vitro quantitative determination of F3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Tissue factor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tissue factor has been precoated onto 96-well plates. Standards(NSO, S33-E251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tissue factor is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tissue factor amount of sample captured in plate. Background: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Human Tissue factor Protein information
Uniprot ID TF_HUMAN
Uniprot AC P13726; D3DT47; Q6FHG2; Q86WH4;
UniGene Hs.62192;
GeneID 2152
KEGG hsa:2152;
Human Tissue factor Protein SEQUENCE
SEQUENCE 295 AA; 33068 MW; D3486C713ED8EAD0 CRC64;
METPAWPRVP RPETAVARTL LLGWVFAQVA GASGTTNTVA AYNLTWKSTN
FKTILEWEPK PVNQVYTVQI STKSGDWKSK CFYTTDTECD LTDEIVKDVK
QTYLARVFSY PAGNVESTGS AGEPLYENSP EFTPYLETNL GQPTIQSFEQ
VGTKVNVTVE DERTLVRRNN TFLSLRDVFG KDLIYTLYYW KSSSSGKKTA
KTNTNEFLID VDKGENYCFS VQAVIPSRTV NRKSTDSPVE CMGQEKGEFR
EIFYIIGAVV FVVIILVIIL AISLHKCRKA GVGQSWKENS PLNVS
UCSC uc001dqr.4; human. [P13726-1];

Human F3 ELISA Kit

Human TF ELISA Kit;Human Coagulation factor III ELISA Kit;Human Thromboplastin ELISA Kit;Human CD142 ELISA Kit;Human TFA ELISA Kit;Human coagulation factor III, tissue factor ELISA Kit;Human tissue factor ELISA Kit;Human coagulation factor III (thromboplastin, tissue factor) ELISA Kit;

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