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Alternative name
Ca++-ATPase ELISA KIT; Ca++-ATPaseOXYTRI_02675 ELISA KIT;
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Catalog
E046442
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species
Mouse
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GeneCa-ATPase
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Other Species
Human Ca (2+) ATPase ELISA KitMouse Ca (2+) -ATPase ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse Ca-ATPase. No significant cross-reactivity or interference between Mouse Ca-ATPase and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse Ca-ATPase and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity18.75pg/mL
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Intended UseMouse Ca-ATPase ELISA Kit allows for the in vitro quantitative determination of Ca-ATPase , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Ca-ATPase in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Ca-ATPase. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse Ca-ATPase and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Ca-ATPase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Ca-ATPase. You can calculate the concentration of Mouse Ca-ATPase in the samples by comparing the OD of the samples to the standard curve.
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