Human AKAP5 ELISA Kit

Alternative name
Human AKAP-5 ELISA Kit;Human A-kinase anchor protein 79 kDa ELISA Kit;Human AKAP 79 ELISA Kit;Human H21 ELISA Kit;Human cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein ELISA Kit;Human AKAP79 ELISA Kit;Human AKAP75 ELISA Kit;Human A-kinase anchoring protein 5 ELISA Kit;Human A-kinase anchor protein 5 ELISA Kit;Human A kinase (PRKA) anchor protein 5 ELISA Kit;Human A-kinase anchoring protein 75/79 ELISA Kit;
Catalog
E000117
species
Human
GeneAKAP5
SpecificityThis assay has high sensitivity and excellent specificity for detection of KAP5. No significant cross-reactivity or interference between KAP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KAP5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman AKAP5 ELISA Kit allows for the in vitro quantitative determination of AKAP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: KAP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KAP5 antibody and an KAP5-HRP conjugate. The assay sample and buffer are incubated together with KAP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KAP5 concentration since KAP5 from samples and KAP5-HRP conjugate compete for the anti-KAP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by KAP5 from the sample, fewer sites are left to bind KAP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KAP5 concentration in each sample is interpolated from this standard curve.
Human A-kinase anchor protein 5 Protein information
Uniprot ID AKAP5_HUMAN
Uniprot AC P24588; A2RRB8;
UniGene Hs.656683;
GeneID 9495
KEGG hsa:9495;
Human A-kinase anchor protein 5 Protein SEQUENCE
SEQUENCE 427 AA; 47088 MW; D2A86298EAF1BE32 CRC64;
METTISEIHV ENKDEKRSAE GSPGAERQKE KASMLCFKRR KKAAKALKPK
AGSEAADVAR KCPQEAGASD QPEPTRGAWA SLKRLVTRRK RSESSKQQKP
LEGEMQPAIN AEDADLSKKK AKSRLKIPCI KFPRGPKRSN HSKIIEDSDC
SIKVQEEAEI LDIQTQTPLN DQATKAKSTQ DLSEGISRKD GDEVCESNVS
NSTTSGEKVI SVELGLDNGH SAIQTGTLIL EEIETIKEKQ DVQPQQASPL
ETSETDHQQP VLSDVPPLPA IPDQQIVEEA SNSTLESAPN GKDYESTEIV
AEETKPKDTE LSQESDFKEN GITEEKSKSE ESKRMEPIAI IITDTEISEF
DVTKSKNVPK QFLISAENEQ VGVFANDNGF EDRTSEQYET LLIETASSLV
KNAIQLSIEQ LVNEMASDDN KINNLLQ
UCSC uc001xhd.4; human.;

Category

Human AKAP5 ELISA Kit