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Alternative name
Complement C4-A ELISA KIT; Acidic complement C4 ELISA KIT; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2C4A ELISA KIT; CO4 ELISA KIT; CPAMD2 ELISA KIT
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Catalog
E049363
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species
Mouse
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GeneC4a
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Other Species
Human C4A ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Complement Component 4a (C4a).
No significant cross-reactivity or interference between Complement Component 4a (C4a) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 13.5pg/mL.
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Intended UseMouse C4a ELISA Kit allows for the in vitro quantitative determination of C4a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4a (C4a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4a (C4a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4a (C4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 4a (C4a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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