Human CYP1A2 ELISA Kit

Alternative name
Human CYPIA2 ELISA Kit;Human cholesterol 25-hydroxylase ELISA Kit;Human cytochrome P(3)450 ELISA Kit;Human cytochrome P450 4 ELISA Kit;Human cytochrome P450-P3 ELISA Kit;Human CP12 ELISA Kit;Human P3-450 ELISA Kit;Human P450(PA) ELISA Kit;Human cytochrome P450 family 1 subfamily A member 2 ELISA Kit;Human cytochrome P450 1A2 ELISA Kit;Human P450 form 4 ELISA Kit;Human aryl hydrocarbon hydroxylase ELISA Kit;Human cytochrome P450, family 1, subfamily A, polypeptide 2 ELISA Kit;Human cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2 ELISA Kit;Human dioxin-inducible P3-450 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human microsomal monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
Catalog
E011018
species
Human
GeneCYP1A2
Standard Curve
Other Species Mouse Cyp1a2 ELISA Kit Mouse CYP1A2(P450) ELISA Kit Canine CYP1A2 ELISA Kit Guinea Pig CYP1A2 ELISA Kit Chicken CYP1A2 ELISA Kit Monkey CYP1A2 ELISA Kit Rabbit CYP1A2 ELISA Kit Rat Cyp1a2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP1A2. No significant cross-reactivity or interference between CYP1A2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CYP1A2 is typically less than 0.051ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by ad
Intended UseHuman CYP1A2 ELISA Kit allows for the in vitro quantitative determination of CYP1A2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP1A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CYP1A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP1A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYP1A2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytochrome P450 1A2 Protein information
Uniprot ID CP1A2_HUMAN
Uniprot AC P05177; Q16754; Q6NWU5; Q9BXX7; Q9UK49;
UniGene Hs.1361;
GeneID 1544
KEGG hsa:1544;
Human Cytochrome P450 1A2 Protein SEQUENCE
SEQUENCE 515 AA; 58294 MW; 23AEFEEBDF6806AB CRC64;
MALSQSVPFS ATELLLASAI FCLVFWVLKG LRPRVPKGLK SPPEPWGWPL
LGHVLTLGKN PHLALSRMSQ RYGDVLQIRI GSTPVLVLSR LDTIRQALVR
QGDDFKGRPD LYTSTLITDG QSLTFSTDSG PVWAARRRLA QNALNTFSIA
SDPASSSSCY LEEHVSKEAK ALISRLQELM AGPGHFDPYN QVVVSVANVI
GAMCFGQHFP ESSDEMLSLV KNTHEFVETA SSGNPLDFFP ILRYLPNPAL
QRFKAFNQRF LWFLQKTVQE HYQDFDKNSV RDITGALFKH SKKGPRASGN
LIPQEKIVNL VNDIFGAGFD TVTTAISWSL MYLVTKPEIQ RKIQKELDTV
IGRERRPRLS DRPQLPYLEA FILETFRHSS FLPFTIPHST TRDTTLNGFY
IPKKCCVFVN QWQVNHDPEL WEDPSEFRPE RFLTADGTAI NKPLSEKMML
FGMGKRRCIG EVLAKWEIFL FLAILLQQLE FSVPPGVKVD LTPIYGLTMK
HARCEHVQAR RFSIN
UCSC uc002ayr.2; human. [P05177-1];

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Human CYP1A2 ELISA Kit