Human CRELD1 ELISA Kit

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  • Alternative name

    Human CIRRIN ELISA Kit;Human AVSD2 ELISA Kit;Human cysteine rich with EGF like domains 1 ELISA Kit;Human cysteine-rich with EGF-like domain protein 1 ELISA Kit;

  • Catalog
    E010839
  • species
    Human
  • GeneCRELD1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cysteine Rich With EGF Like Domains Protein 1 (CRELD1). No significant cross-reactivity or interference between Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.113ng/mL.
  • Intended UseHuman CRELD1 ELISA Kit allows for the in vitro quantitative determination of CRELD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich With EGF Like Domains Protein 1 (CRELD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich With EGF Like Domains Protein 1 (CRELD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich With EGF Like Domains Protein 1 (CRELD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cysteine-rich with EGF-like domain protein 1 Protein information
  • Uniprot ID CREL1_HUMAN
  • Uniprot AC Q96HD1; A8MX90; B2RAA9; Q6I9X5; Q8NFT4; Q9Y409;
  • UniGene Hs.9383;
  • GeneID 78987
  • KEGG hsa:78987;
  • Human Cysteine-rich with EGF-like domain protein 1 Protein SEQUENCE
  • SEQUENCE 420 AA; 45440 MW; EEAAD0D143A0D35A CRC64;

    MAPWPPKGLV PAMLWGLSLF LNLPGPIWLQ PSPPPQSSPP PQPHPCHTCR

    GLVDSFNKGL ERTIRDNFGG GNTAWEEENL SKYKDSETRL VEVLEGVCSK

    SDFECHRLLE LSEELVESWW FHKQQEAPDL FQWLCSDSLK LCCPAGTFGP

    SCLPCPGGTE RPCGGYGQCE GEGTRGGSGH CDCQAGYGGE ACGQCGLGYF

    EAERNASHLV CSACFGPCAR CSGPEESNCL QCKKGWALHH LKCVDIDECG

    TEGANCGADQ FCVNTEGSYE CRDCAKACLG CMGAGPGRCK KCSPGYQQVG

    SKCLDVDECE TEVCPGENKQ CENTEGGYRC ICAEGYKQME GICVKEQIPE

    SAGFFSEMTE DELVVLQQMF FGIIICALAT LAAKGDLVFT AIFIGAVAAM

    TGYWLSERSD RVLEGFIKGR

  • UCSC uc003buf.4; human. [Q96HD1-1];



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