Human CFLAR ELISA Kit

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  • Alternative name

    Human caspase homolog ELISA Kit;Human CASH ELISA Kit;Human caspase-eight-related protein ELISA Kit;Human Casper ELISA Kit;Human caspase-like apoptosis regulatory protein ELISA Kit;Human CLARP ELISA Kit;Human cellular FLICE-like inhibitory protein ELISA Kit;Human c-FLIP ELISA Kit;Human FADD-like antiapoptotic molecule 1 ELISA Kit;Human FLAME-1 ELISA Kit;Human inhibitor of FLICE ELISA Kit;Human I-FLICE ELISA Kit;Human MACH-related inducer of toxicity ELISA Kit;Human MRIT ELISA Kit;Human Usurpin ELISA Kit;Human CASP8AP1 ELISA Kit;Human FLAME ELISA Kit;Human FLAME1 ELISA Kit;Human FLIP ELISA Kit;Human c-FLIPL ELISA Kit;Human c-FLIPR ELISA Kit;Human c-FLIPS ELISA Kit;Human CASP8 and FADD like apoptosis regulator ELISA Kit;Human CASP8 and FADD-like apoptosis regulator ELISA Kit;Human caspase-related inducer of apoptosis ELISA Kit;Human testis secretory sperm-binding protein Li 233m ELISA Kit;Human usurpin beta ELISA Kit;

  • Catalog
    E006837
  • species
    Human
  • GeneCFLAR
  • Standard CurveHuman CFLAR ELISA Kit
  • Other Species Mouse Cflar ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CFLAR. No significant cross-reactivity or interference between CFLAR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CFLAR and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CFLAR ELISA Kit allows for the in vitro quantitative determination of CFLAR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: CFLAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFLAR antibody and an CFLAR-HRP conjugate. The assay sample and buffer are incubated together with CFLAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFLAR concentration since CFLAR from samples and CFLAR-HRP conjugate compete for the anti-CFLAR antibody binding site. Since the number of sites is limited, as more sites are occupied by CFLAR from the sample, fewer sites are left to bind CFLAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFLAR concentration in each sample is interpolated from this standard curve.
  • Human CASP8 and FADD-like apoptosis regulator subunit p12 Protein information
  • Uniprot ID CFLAR_HUMAN
  • Uniprot AC Q54AF1; Q96TE4; Q9UEW1;
  • UniGene Hs.390736; Hs.731912;
  • GeneID 8837
  • KEGG hsa:8837;
  • Human CASP8 and FADD-like apoptosis regulator subunit p12 Protein SEQUENCE
  • SEQUENCE 480 AA; 55344 MW; 8C6D7E92AE1EB672 CRC64;

    MSAEVIHQVE EALDTDEKEM LLFLCRDVAI DVVPPNVRDL LDILRERGKL

    SVGDLAELLY RVRRFDLLKR ILKMDRKAVE THLLRNPHLV SDYRVLMAEI

    GEDLDKSDVS SLIFLMKDYM GRGKISKEKS FLDLVVELEK LNLVAPDQLD

    LLEKCLKNIH RIDLKTKIQK YKQSVQGAGT SYRNVLQAAI QKSLKDPSNN

    FRLHNGRSKE QRLKEQLGAQ QEPVKKSIQE SEAFLPQSIP EERYKMKSKP

    LGICLIIDCI GNETELLRDT FTSLGYEVQK FLHLSMHGIS QILGQFACMP

    EHRDYDSFVC VLVSRGGSQS VYGVDQTHSG LPLHHIRRMF MGDSCPYLAG

    KPKMFFIQNY VVSEGQLEDS SLLEVDGPAM KNVEFKAQKR GLCTVHREAD

    FFWSLCTADM SLLEQSHSSP SLYLQCLSQK LRQERKRPLL DLHIELNGYM

    YDWNSRVSAK EKYYVWLQHT LRKKLILSYT

  • UCSC uc002uwz.4; human. [O15519-1];


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