Human CFLAR ELISA Kit

Alternative name
Human caspase homolog ELISA Kit;Human CASH ELISA Kit;Human caspase-eight-related protein ELISA Kit;Human Casper ELISA Kit;Human caspase-like apoptosis regulatory protein ELISA Kit;Human CLARP ELISA Kit;Human cellular FLICE-like inhibitory protein ELISA Kit;Human c-FLIP ELISA Kit;Human FADD-like antiapoptotic molecule 1 ELISA Kit;Human FLAME-1 ELISA Kit;Human inhibitor of FLICE ELISA Kit;Human I-FLICE ELISA Kit;Human MACH-related inducer of toxicity ELISA Kit;Human MRIT ELISA Kit;Human Usurpin ELISA Kit;Human CASP8AP1 ELISA Kit;Human FLAME ELISA Kit;Human FLAME1 ELISA Kit;Human FLIP ELISA Kit;Human c-FLIPL ELISA Kit;Human c-FLIPR ELISA Kit;Human c-FLIPS ELISA Kit;Human CASP8 and FADD like apoptosis regulator ELISA Kit;Human CASP8 and FADD-like apoptosis regulator ELISA Kit;Human caspase-related inducer of apoptosis ELISA Kit;Human testis secretory sperm-binding protein Li 233m ELISA Kit;Human usurpin beta ELISA Kit;
Catalog
E006837
species
Human
GeneCFLAR
SpecificityThis assay has high sensitivity and excellent specificity for detection of CFLAR. No significant cross-reactivity or interference between CFLAR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CFLAR and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CFLAR ELISA Kit allows for the in vitro quantitative determination of CFLAR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CFLAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFLAR antibody and an CFLAR-HRP conjugate. The assay sample and buffer are incubated together with CFLAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFLAR concentration since CFLAR from samples and CFLAR-HRP conjugate compete for the anti-CFLAR antibody binding site. Since the number of sites is limited, as more sites are occupied by CFLAR from the sample, fewer sites are left to bind CFLAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFLAR concentration in each sample is interpolated from this standard curve.
Human CASP8 and FADD-like apoptosis regulator subunit p12 Protein information
Uniprot ID CFLAR_HUMAN
Uniprot AC Q54AF1; Q96TE4; Q9UEW1;
UniGene Hs.390736; Hs.731912;
GeneID 8837
KEGG hsa:8837;
Human CASP8 and FADD-like apoptosis regulator subunit p12 Protein SEQUENCE
SEQUENCE 480 AA; 55344 MW; 8C6D7E92AE1EB672 CRC64;
MSAEVIHQVE EALDTDEKEM LLFLCRDVAI DVVPPNVRDL LDILRERGKL
SVGDLAELLY RVRRFDLLKR ILKMDRKAVE THLLRNPHLV SDYRVLMAEI
GEDLDKSDVS SLIFLMKDYM GRGKISKEKS FLDLVVELEK LNLVAPDQLD
LLEKCLKNIH RIDLKTKIQK YKQSVQGAGT SYRNVLQAAI QKSLKDPSNN
FRLHNGRSKE QRLKEQLGAQ QEPVKKSIQE SEAFLPQSIP EERYKMKSKP
LGICLIIDCI GNETELLRDT FTSLGYEVQK FLHLSMHGIS QILGQFACMP
EHRDYDSFVC VLVSRGGSQS VYGVDQTHSG LPLHHIRRMF MGDSCPYLAG
KPKMFFIQNY VVSEGQLEDS SLLEVDGPAM KNVEFKAQKR GLCTVHREAD
FFWSLCTADM SLLEQSHSSP SLYLQCLSQK LRQERKRPLL DLHIELNGYM
YDWNSRVSAK EKYYVWLQHT LRKKLILSYT
UCSC uc002uwz.4; human. [O15519-1];

Category

Human CFLAR ELISA Kit