Human RFK ELISA Kit

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  • Alternative name

    Human ATP:riboflavin 5'-phosphotransferase ELISA Kit;Human flavokinase ELISA Kit;Human RIFK ELISA Kit;Human riboflavin kinase ELISA Kit;Human 0610038L10Rik ELISA Kit;

  • Catalog
    E033582
  • species
    Human
  • GeneRFK
  • Standard CurveHuman RFK ELISA Kit
  • Other Species Mouse Rfk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Riboflavin Kinase (RFK). No significant cross-reactivity or interference between Riboflavin Kinase (RFK) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseHuman RFK ELISA Kit allows for the in vitro quantitative determination of RFK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Riboflavin Kinase (RFK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Riboflavin Kinase (RFK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Riboflavin Kinase (RFK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Riboflavin Kinase (RFK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Riboflavin kinase Protein information
  • Uniprot ID RIFK_HUMAN
  • Uniprot AC Q969G6; Q5JSG9; Q9NUT7;
  • UniGene Hs.37558;
  • GeneID 55312
  • KEGG hsa:55312;
  • Human Riboflavin kinase Protein SEQUENCE
  • SEQUENCE 155 AA; 17623 MW; 3E038E487E164EBA CRC64;

    MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW

    ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV

    GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK

    IMNGH

  • UCSC uc004akd.3; human.;


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