Intended UseHorse 25-HVD3 ELISA Kit allows for the in vitro quantitative determination of 25-HVD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole kit at 4℃
Product Description specificalHorse 25-HVD3 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 25-HVD3 , During the reaction,25-HVD3 in the sample or standard competes with a fixed amount of biotin-labeled 25-HVD3 for sites on a pre-coated Monoclonal antibody specific to 25-HVD3 . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 25-HVD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.