Human ATF7 ELISA Kit

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  • Alternative name

    Human cAMP-dependent transcription factor ATF-7 ELISA Kit;Human activating transcription factor 7 ELISA Kit;Human transcription factor ATF-A ELISA Kit;Human ATFA ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-7 ELISA Kit;

  • Catalog
    E003932
  • species
    Human
  • GeneATF7
  • Other Species Mouse Atf7 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ATF7. No significant cross-reactivity or interference between Human ATF7 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ATF7 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman ATF7 ELISA Kit allows for the in vitro quantitative determination of ATF7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATF7 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATF7. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ATF7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATF7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ATF7. You can calculate the concentration of Human ATF7 in the samples by comparing the OD of the samples to the standard curve.
  • Human Cyclic AMP-dependent transcription factor ATF-7 Protein information
  • Uniprot ID ATF7_HUMAN
  • Uniprot AC P17544; A5D6Y4; B2RMP1; B4DQL4; Q13814; Q8IVR8; Q9UD83;
  • UniGene Hs.12286; Hs.633602;
  • GeneID 11016
  • KEGG hsa:11016;
  • Human Cyclic AMP-dependent transcription factor ATF-7 Protein SEQUENCE
  • SEQUENCE 494 AA; 52967 MW; 511EAF79C6503300 CRC64;

    MGDDRPFVCN APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RTDSVIIADQ

    TPTPTRFLKN CEEVGLFNEL ASSFEHEFKK AADEDEKKAR SRTVAKKLVA

    AAGPLDMSLP STPDIKIKEE EPVEVDSSPP DSPASSPCSP PLKEKEVTPK

    PVLISTPTPT IVRPGSLPLH LGYDPLHPTL PSPTSVITQA PPSNRQMGSP

    TGSLPLVMHL ANGQTMPVLP GPPVQMPSVI SLARPVSMVP NIPGIPGPPV

    NSSGSISPSG HPIPSEAKMR LKATLTHQVS SINGGCGMVV GTASTMVTAR

    PEQSQILIQH PDAPSPAQPQ VSPAQPTPST GGRRRRTVDE DPDERRQRFL

    ERNRAAASRC RQKRKLWVSS LEKKAEELTS QNIQLSNEVT LLRNEVAQLK

    QLLLAHKDCP VTALQKKTQG YLESPKESSE PTGSPAPVIQ HSSATAPSNG

    LSVRSAAEAV ATSVLTQMAS QRTELSMPIQ SHVIMTPQSQ SAGR

  • UCSC uc001sdz.4; human. [P17544-1];



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