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Alternative name
Calcitriol ELISA Kit,Calcitriol ELISA Kit,1 ELISA Kit,25-dihydroxycholecalciferol ELISA Kit,1 ELISA Kit,25-dihydroxyvitamin D3 ELISA Kit
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Catalog
E104219
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species
Mouse
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GeneCalcitriol
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Standard Curve
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Other Species
Bovine Calcitriol ELISA KitCanine Calcitriol ELISA KitChicken Calcitriol ELISA KitHuman Calcitriol ELISA KitGeneral Calcitriol ELISA KitPorcine Calcitriol ELISA KitRabbit Calcitriol ELISA KitRat Calcitriol ELISA KitCamel Calcitriol ELISA KitGoat Calcitriol ELISA KitGuinea Pig Calcitriol ELISA KitHamster Calcitriol ELISA KitHorse Calcitriol ELISA KitMonkey Calcitriol ELISA KitSheep Calcitriol ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity10pg/ml
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Intended UseMouse Calcitriol ELISA Kit allows for the in vitro quantitative determination of Calcitriol , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalMouse Calcitriol ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to Calcitriol, During the reaction,Calcitriol in the sample or standard competes with a fixed amount of biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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