Human BCL6 ELISA Kit

Alternative name
Human BCL-6 ELISA Kit;Human B-cell lymphoma 5 protein ELISA Kit;Human BCL-5 ELISA Kit;Human protein LAZ-3 ELISA Kit;Human zinc finger and BTB domain-containing protein 27 ELISA Kit;Human zinc finger protein 51 ELISA Kit;Human BCL5 ELISA Kit;Human LAZ3 ELISA Kit;Human ZBTB27 ELISA Kit;Human ZNF51 ELISA Kit;Human BCL6A ELISA Kit;Human B-cell CLL/lymphoma 6 ELISA Kit;Human B-cell lymphoma 6 protein ELISA Kit;Human B-cell lymphoma 6 protein transcript ELISA Kit;Human cys-his2 zinc finger transcription factor ELISA Kit;Human lymphoma-associated zinc finger gene on chromosome 3 ELISA Kit;Human zinc finger transcription factor BCL6S ELISA Kit;
Catalog
E004319
species
Human
GeneBCL6
Standard Curve
Other Species Mouse Bcl6 ELISA Kit Chicken BCL6 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of BCL6. No significant cross-reactivity or interference between BCL6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL6 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman BCL6 ELISA Kit allows for the in vitro quantitative determination of BCL6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: BCL6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL6 antibody and an BCL6-HRP conjugate. The assay sample and buffer are incubated together with BCL6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL6 concentration since BCL6 from samples and BCL6-HRP conjugate compete for the anti-BCL6 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL6 from the sample, fewer sites are left to bind BCL6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL6 concentration in each sample is interpolated from this standard curve.
Human B-cell lymphoma 6 protein Protein information
Uniprot ID BCL6_HUMAN
Uniprot AC P41182; A7E241; B8PSA7; D3DNV5;
UniGene Hs.478588;
GeneID 604
KEGG hsa:604;
Human B-cell lymphoma 6 protein Protein SEQUENCE
SEQUENCE 706 AA; 78846 MW; E38D83C213DAE2D0 CRC64;
MASPADSCIQ FTRHASDVLL NLNRLRSRDI LTDVVIVVSR EQFRAHKTVL
MACSGLFYSI FTDQLKCNLS VINLDPEINP EGFCILLDFM YTSRLNLREG
NIMAVMATAM YLQMEHVVDT CRKFIKASEA EMVSAIKPPR EEFLNSRMLM
PQDIMAYRGR EVVENNLPLR SAPGCESRAF APSLYSGLST PPASYSMYSH
LPVSSLLFSD EEFRDVRMPV ANPFPKERAL PCDSARPVPG EYSRPTLEVS
PNVCHSNIYS PKETIPEEAR SDMHYSVAEG LKPAAPSARN APYFPCDKAS
KEEERPSSED EIALHFEPPN APLNRKGLVS PQSPQKSDCQ PNSPTESCSS
KNACILQASG SPPAKSPTDP KACNWKKYKF IVLNSLNQNA KPEGPEQAEL
GRLSPRAYTA PPACQPPMEP ENLDLQSPTK LSASGEDSTI PQASRLNNIV
NRSMTGSPRS SSESHSPLYM HPPKCTSCGS QSPQHAEMCL HTAGPTFPEE
MGETQSEYSD SSCENGAFFC NECDCRFSEE ASLKRHTLQT HSDKPYKCDR
CQASFRYKGN LASHKTVHTG EKPYRCNICG AQFNRPANLK THTRIHSGEK
PYKCETCGAR FVQVAHLRAH VLIHTGEKPY PCEICGTRFR HLQTLKSHLR
IHTGEKPYHC EKCNLHFRHK SQLRLHLRQK HGAITNTKVQ YRVSATDLPP
ELPKAC
UCSC uc003frp.4; human. [P41182-1];

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