Bovine F-E3 ELISA Kit

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  • Alternative name

    Free Estriol ELISA Kit

  • Catalog
  • species
  • GeneF-E3
  • Standard CurveBovine F-E3 ELISA Kit
  • Other Species Canine F-E3 ELISA KitChicken F-E3 ELISA KitHuman F-E3 ELISA KitGeneral F-E3 ELISA KitMouse F-E3 ELISA KitPorcine F-E3 ELISA KitRabbit F-E3 ELISA KitRat F-E3 ELISA KitCamel F-E3 ELISA KitGoat F-E3 ELISA KitGuinea Pig F-E3 ELISA KitHamster F-E3 ELISA KitHorse F-E3 ELISA KitMonkey F-E3 ELISA KitSheep F-E3 ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.375ng/ml
  • Intended UseBovine F-E3 ELISA Kit allows for the in vitro quantitative determination of F-E3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    Bovine F-E3 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to F-E3, During the reaction,F-E3 in the sample or standard competes with a fixed amount of biotin-labeled F-E3 for sites on a pre-coated Monoclonal antibody specific to F-E3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of F-E3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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