Human p21 ELISA Kit

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  • Alternative name

    Cyclin-dependent kinase inhibitor 1 ELISA KIT; CDK-interacting protein 1 ELISA KIT; Melanoma differentiation-associated protein 6 ELISA KIT; MDA-6 ELISA KIT; p21CDKN1A ELISA KIT; CAP20 ELISA KIT; CDKN1 ELISA KIT; CIP1 ELISA KIT; MDA6 ELISA KIT; PIC1 ELISA KIT; SDI1 ELISA KIT; WAF1 ELISA KIT; MDA-6 ELISA KIT

  • Catalog
    E003132
  • species
    Human
  • Genep21
  • Other Species Mouse p21 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman p21 ELISA Kit allows for the in vitro quantitative determination of p21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the Assay: p21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-p21 antibody and an p21-HRP conjugate. The assay sample and buffer are incubated together with p21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p21 concentration since p21 from samples and p21-HRP conjugate compete for the anti-p21 antibody binding site. Since the number of sites is limited, as more sites are occupied by p21 from the sample, fewer sites are left to bind p21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p21 concentration in each sample is interpolated from this standard curve.




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