SpecificityThis kit recognizes natural and recombinant PDHbeta. No significant cross-reactivity or interference between PDHbeta and analogues was observed.
SamplesSerum, plasma and other biological fluids.
SensitivityThe minimum detectable dose of PDHbeta is 0.188mU/mL
Product Description specificalIntended Uses||This CLIA kit applies to the in vitro quantitative determination of PDHbeta concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PDHbeta. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDHbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDHbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PDHbeta. You can calculate the concentration of PDHbeta in the samples by comparing the RLU value of the samples to the standard curve.