Human GDF7 ELISA Kit

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  • Alternative name

    Human GDF-7 ELISA Kit;Human BMP12 ELISA Kit;Human growth differentiation factor 7 ELISA Kit;Human growth/differentiation factor 7 ELISA Kit;

  • Catalog
    E017545
  • species
    Human
  • GeneGDF7
  • Standard CurveHuman GDF7 ELISA Kit
  • Other Species Mouse Gdf7 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GDF7. No significant cross-reactivity or interference between GDF7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF7 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman GDF7 ELISA Kit allows for the in vitro quantitative determination of GDF7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the assay: GDF7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF7 antibody and an GDF7-HRP conjugate. The assay sample and buffer are incubated together with GDF7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF7 concentration since GDF7 from samples and GDF7-HRP conjugate compete for the anti-GDF7 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF7 from the sample, fewer sites are left to bind GDF7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF7 concentration in each sample is interpolated from this standard curve.
  • Human Growth/differentiation factor 7 Protein information
  • Uniprot ID GDF7_HUMAN
  • Uniprot AC Q7Z4P5;
  • UniGene Hs.447688; Hs.596282;
  • GeneID 151449
  • KEGG hsa:151449;
  • Human Growth/differentiation factor 7 Protein SEQUENCE
  • SEQUENCE 450 AA; 46950 MW; C7D4D417685D25BD CRC64;

    MDLSAAAALC LWLLSACRPR DGLEAAAVLR AAGAGPVRSP GGGGGGGGGG

    RTLAQAAGAA AVPAAAVPRA RAARRAAGSG FRNGSVVPHH FMMSLYRSLA

    GRAPAGAAAV SASGHGRADT ITGFTDQATQ DESAAETGQS FLFDVSSLND

    ADEVVGAELR VLRRGSPESG PGSWTSPPLL LLSTCPGAAR APRLLYSRAA

    EPLVGQRWEA FDVADAMRRH RREPRPPRAF CLLLRAVAGP VPSPLALRRL

    GFGWPGGGGS AAEERAVLVV SSRTQRKESL FREIRAQARA LGAALASEPL

    PDPGTGTASP RAVIGGRRRR RTALAGTRTA QGSGGGAGRG HGRRGRSRCS

    RKPLHVDFKE LGWDDWIIAP LDYEAYHCEG LCDFPLRSHL EPTNHAIIQT

    LLNSMAPDAA PASCCVPARL SPISILYIDA ANNVVYKQYE DMVVEACGCR

  • UCSC uc002rdz.2; human.;


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