Human AGK ELISA Kit

Alternative name
Human hAGK ELISA Kit;Human multiple substrate lipid kinase ELISA Kit;Human hsMuLK ELISA Kit;Human MULK ELISA Kit;Human multi-substrate lipid kinase ELISA Kit;Human CATC5 ELISA Kit;Human CTRCT38 ELISA Kit;Human MTDPS10 ELISA Kit;Human acylglycerol kinase ELISA Kit;Human acylglycerol kinase, mitochondrial ELISA Kit;
Catalog
E000640
species
Human
GeneAGK
Standard Curve
Other Species Mouse Agk ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Acylglycerol Kinase (AGK). No significant cross-reactivity or interference between Acylglycerol Kinase (AGK) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.081ng/mL.
Intended UseHuman AGK ELISA Kit allows for the in vitro quantitative determination of AGK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acylglycerol Kinase (AGK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acylglycerol Kinase (AGK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acylglycerol Kinase (AGK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acylglycerol Kinase (AGK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Acylglycerol kinase, mitochondrial Protein information
Uniprot ID AGK_HUMAN
Uniprot AC Q53H12; Q75KN1; Q96GC3; Q9NP48;
UniGene Hs.743318;
GeneID 55750
KEGG hsa:55750;
Human Acylglycerol kinase, mitochondrial Protein SEQUENCE
SEQUENCE 422 AA; 47137 MW; F9D85658616B8970 CRC64;
MTVFFKTLRN HWKKTTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFG
NQLIPPNAQV KKATVFLNPA ACKGKARTLF EKNAAPILHL SGMDVTIVKT
DYEGQAKKLL ELMENTDVII VAGGDGTLQE VVTGVLRRTD EATFSKIPIG
FIPLGETSSL SHTLFAESGN KVQHITDATL AIVKGETVPL DVLQIKGEKE
QPVFAMTGLR WGSFRDAGVK VSKYWYLGPL KIKAAHFFST LKEWPQTHQA
SISYTGPTER PPNEPEETPV QRPSLYRRIL RRLASYWAQP QDALSQEVSP
EVWKDVQLST IELSITTRNN QLDPTSKEDF LNICIEPDTI SKGDFITIGS
RKVRNPKLHV EGTECLQASQ CTLLIPEGAG GSFSIDSEEY EAMPVEVKLL
PRKLQFFCDP RKREQMLTSP TQ
UCSC uc003vwi.3; human. [Q53H12-1];

Category

Human AGK ELISA Kit