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Catalog
C003793
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GeneAngII
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.
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SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Detection Range11.72-3000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 5.17pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Angiotensin II (AngII) and unlabeled Angiotensin II (AngII) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin II (AngII) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Angiotensin II (AngII) level in the sample or standard.
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