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Alternative name
Histone H2A.Z ELISA KIT; H2AFZ ELISA KIT; H2AZ ELISA KIT; H2A/z ELISA KIT
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Catalog
E044238
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species
Mouse
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GeneAHA
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SpecificityThis assay has high sensitivity and excellent specificity for detection of HISAb. No significant cross-reactivity or interference between HISAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HISAb and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ug/ml.
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Intended UseMouse AHA ELISA Kit allows for the in vitro quantitative determination of AHA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalIntended Uses: This HISAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HISAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||HISAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Histone antigen and an HISAb-HRP conjugate. The assay sample and buffer are incubated together with HISAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HISAb concentration since HISAb from samples and HISAb-HRP conjugate compete for the Histone antigen binding site. Since the number of sites is limited, as more sites are occupied by HISAb from the sample, fewer sites are left to bind HISAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HISAb concentration in each sample is interpolated from this standard curve.
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