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Mouse Atp1b1 ELISA Kit

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  • Alternative name

    Mouse sodium/potassium-dependent ATPase subunit beta-1 ELISA Kit;Mouse Atp4b ELISA Kit;Mouse Atpb ELISA Kit;Mouse Atpb-1 ELISA Kit;Mouse NKbeta1 ELISA Kit;Mouse ATPase, Na+/K+ transporting, beta 1 polypeptide ELISA Kit;Mouse sodium/potassium-transporting ATPase subunit beta-1 ELISA Kit;Mouse sodium/potassium ATPase beta subunit ELISA Kit;
  • Catalog
    E045254
  • species
    Mouse
  • GeneAtp1b1
  • Standard CurveMouse Atp1b1 ELISA Kit
  • Other Species Human ATP1B1 ELISA KitCanine ATP1B1 ELISA KitGuinea Pig ATP1B1 ELISA KitChicken ATP1B1 ELISA KitMonkey ATP1B1 ELISA KitPorcine ATP1B1 ELISA KitRabbit ATP1B1 ELISA KitRat Atp1b1 ELISA KitSheep ATP1B1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.071ng/mL.
  • Intended UseMouse Atp1b1 ELISA Kit allows for the in vitro quantitative determination of Atp1b1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Sodium/potassium-transporting ATPase subunit beta-1 Protein information
  • Uniprot ID AT1B1_MOUSE
  • Uniprot AC P14094;
  • UniGene Mm.4550;
  • GeneID 11931
  • KEGG mmu:11931;
  • Mouse Sodium/potassium-transporting ATPase subunit beta-1 Protein SEQUENCE

  • SEQUENCE 304 AA; 35195 MW; 8E15D8F860932680 CRC64;

    MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF

    IGTIQVMLLT ISELKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA

    YVLNIIRFLE KYKDSAQKDD MIFEDCGNVP SEPKERGDIN HERGERKVCR

    FKLDWLGNCS GLNDDSYGYR EGKPCIIIKL NRVLGFKPKP PKNESLETYP

    LMMKYNPNVL PVQCTGKRDE DKDKVGNIEY FGMGGYYGFP LQYYPYYGKL

    LQPKYLQPLL AVQFTNLTVD TEIRVECKAY GENIGYSEKD RFQGRFDVKI

    EIKS

  • UCSC uc007dip.2; mouse.;


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