Human TG clia kit

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  • Catalog
  • Alternative name

    Thyroglobulin CLIA KIT; TG CLIA KIT; Tg CLIA KIT

  • GeneTG
  • Other Species Human TG clia kitRat TG clia kitMouse TG clia kitHuman b-TG clia kitRat b-TG clia kitMouse b-TG clia kit
  • Species ReactivityHuman
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of thyroglobulin. No significant cross-reactivity or interference between thyroglobulin and analogues was observed.
  • Detection Range68.6-50,000pg/mL. The standard curve concentrations used for the CLIA's were 50,000pg/mL, 16,666.7pg/mL, 5,555.6pg/mL, 1,851.9pg/mL, 617.3pg/mL, 205.8pg/mL, 68.6pg/mL.
  • SensitivityThe minimum detectable dose of thyroglobulin is typically less than 23.6pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
  • Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to thyroglobulin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to thyroglobulin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the thyroglobulin level in the sample or standard.

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