Mouse Stat4 ELISA Kit

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  • Alternative name

    Mouse signal transducer and activator of transcription 4 ELISA Kit;

  • Catalog
    E067792
  • species
    Mouse
  • GeneStat4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseMouse Stat4 ELISA Kit allows for the in vitro quantitative determination of Stat4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse STAT4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse STAT4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse STAT4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse STAT4 can be calculated.
  • Mouse Signal transducer and activator of transcription 4 Protein information
  • Uniprot ID STAT4_MOUSE
  • Uniprot AC P42228;
  • UniGene Mm.1550;
  • GeneID 20849
  • KEGG mmu:20849;
  • Mouse Signal transducer and activator of transcription 4 Protein SEQUENCE
  • SEQUENCE 749 AA; 85941 MW; A88B837E49FCFEBC CRC64;

    MSQWNQVQQL EIKFLEQVDQ FYDDNFPMEI RHLLAQWIET QDWEVASNNE

    TMATILLQNL LIQLDEQLGR VSKEKNLLLI HNLKRIRKVL QGKFHGNPMH

    VAVVISNCLR EERRILAAAN MPIQGPLEKS LQSSSVSERQ RNVEHKVSAI

    KNSVQMTEQD TKYLEDLQDE FDYRYKTIQT MDQGDKNSIL VNQEVLTLLQ

    EMLNSLDFKR KEALSKMTQI VNETDLLMNS MLLEELQDWK KRQQIACIGG

    PLHNGLDQLQ NCFTLLAESL FQLRQQLEKL QEQSTKMTYE GDPIPAQRAH

    LLERATFLIY NLFKNSFVVE RQPCMPTHPQ RPMVLKTLIQ FTVKLRLLIK

    LPELNYQVKV KASIDKNVST LSNRRFVLCG THVKAMSSEE SSNGSLSVEF

    RHLQPKEMKC STGSKGNEGC HMVTEELHSI TFETQICLYG LTINLETSSL

    PVVMISNVSQ LPNAWASIIW YNVSTNDSQN LVFFNNPPSV TLGQLLEVMS

    WQFSSYVGRG LNSEQLNMLA EKLTVQSNYN DGHLTWAKFC KEHLPGKTFT

    FWTWLEAILD LIKKHILPLW IDGYIMGFVS KEKERLLLKD KMPGTFLLRF

    SESHLGGITF TWVDQSENGE VRFHSVEPYN KGRLSALAFA DILRDYKVIM

    AENIPENPLK YLYPDIPKDK AFGKHYSSQP CEVSRPTERG DKGYVPSVFI

    PISTIRSDST EPQSPSDLLP MSPSAYAVLR ENLSPTTIET AMNSPYSAE




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