Human KRT12 ELISA Kit

Alternative name
Human cytokeratin-12 ELISA Kit;Human CK-12 ELISA Kit;Human Keratin-12 ELISA Kit;Human K12 ELISA Kit;Human keratin 12 ELISA Kit;Human keratin, type I cytoskeletal 12 ELISA Kit;Human keratin 12, type I ELISA Kit;
Catalog
E021436
species
Human
GeneKRT12
Standard Curve
Other Species Mouse Krt12 ELISA Kit Rabbit KRT12 ELISA Kit Rat Krt12 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Keratin 12 (KRT12). No significant cross-reactivity or interference between Keratin 12 (KRT12) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman KRT12 ELISA Kit allows for the in vitro quantitative determination of KRT12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 12 (KRT12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 12 (KRT12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 12 (KRT12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 12 (KRT12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Keratin, type I cytoskeletal 12 Protein information
Uniprot ID K1C12_HUMAN
Uniprot AC Q99456; B2R9E0;
UniGene Hs.66739;
GeneID 3859
KEGG hsa:3859;
Human Keratin, type I cytoskeletal 12 Protein SEQUENCE
SEQUENCE 494 AA; 53511 MW; 75C981380532B682 CRC64;
MDLSNNTMSL SVRTPGLSRR LSSQSVIGRP RGMSASSVGS GYGGSAFGFG
ASCGGGFSAA SMFGSSSGFG GGSGSSMAGG LGAGYGRALG GGSFGGLGMG
FGGSPGGGSL GILSGNDGGL LSGSEKETMQ NLNDRLASYL DKVRALEEAN
TELENKIREW YETRGTGTAD ASQSDYSKYY PLIEDLRNKI ISASIGNAQL
LLQIDNARLA AEDFRMKYEN ELALRQGVEA DINGLRRVLD ELTLTRTDLE
MQIESLNEEL AYMKKNHEDE LQSFRVGGPG EVSVEMDAAP GVDLTRLLND
MRAQYETIAE QNRKDAEAWF IEKSGELRKE ISTNTEQLQS SKSEVTDLRR
AFQNLEIELQ SQLAMKKSLE DSLAEAEGDY CAQLSQVQQL ISNLEAQLLQ
VRADAERQNV DHQRLLNVKA RLELEIETYR RLLDGEAQGD GLEESLFVTD
SKSQAQSTDS SKDPTKTRKI KTVVQEMVNG EVVSSQVQEI EELM
UCSC uc002hvk.3; human.;

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Category

Human KRT12 ELISA Kit

Human cytokeratin-12 ELISA Kit;Human CK-12 ELISA Kit;Human Keratin-12 ELISA Kit;Human K12 ELISA Kit;Human keratin 12 ELISA Kit;Human keratin, type I cytoskeletal 12 ELISA Kit;Human keratin 12, type I ELISA Kit;