Human PDE5A ELISA Kit

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  • Alternative name

    Human cGMP-binding cGMP-specific phosphodiesterase ELISA Kit;Human CGB-PDE ELISA Kit;Human PDE5 ELISA Kit;Human CN5A ELISA Kit;Human phosphodiesterase 5A ELISA Kit;Human cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit;Human cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase ELISA Kit;Human cGMP-specific phosphodiesterase PDE5A2 ELISA Kit;Human cGMP-specific phosphodiesterase type 5A ELISA Kit;Human phosphodiesterase 5A, cGMP-specific ELISA Kit;Human phosphodiesterase isozyme 5 ELISA Kit;

  • Catalog
    E029043
  • species
    Human
  • GenePDE5A
  • Standard CurveHuman PDE5A ELISA Kit
  • Other Species Mouse Pde5a ELISA KitBovine PDE5A ELISA KitCanine PDE5A ELISA KitRat Pde5a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 5A, cGMP Specific (PDE5A). No significant cross-reactivity or interference between Phosphodiesterase 5A, cGMP Specific (PDE5A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.59ng/mL.
  • Intended UseHuman PDE5A ELISA Kit allows for the in vitro quantitative determination of PDE5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 5A, cGMP Specific (PDE5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 5A, cGMP Specific (PDE5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 5A, cGMP Specific (PDE5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 5A, cGMP Specific (PDE5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human cGMP-specific 3',5'-cyclic phosphodiesterase Protein information
  • Uniprot ID PDE5A_HUMAN
  • Uniprot AC O76074; A0AV69; A8K2C4; O75026; O75887; Q86UI0; Q86V66; Q9Y6Z6;
  • UniGene Hs.647971;
  • GeneID 8654
  • KEGG hsa:8654;
  • Human cGMP-specific 3',5'-cyclic phosphodiesterase Protein SEQUENCE
  • SEQUENCE 875 AA; 99985 MW; 9E30C6C182F13388 CRC64;

    MERAGPSFGQ QRQQQQPQQQ KQQQRDQDSV EAWLDDHWDF TFSYFVRKAT

    REMVNAWFAE RVHTIPVCKE GIRGHTESCS CPLQQSPRAD NSAPGTPTRK

    ISASEFDRPL RPIVVKDSEG TVSFLSDSEK KEQMPLTPPR FDHDEGDQCS

    RLLELVKDIS SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSNDKFL

    ISRLFDVAEG STLEEVSNNC IRLEWNKGIV GHVAALGEPL NIKDAYEDPR

    FNAEVDQITG YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE

    KDFAAYLAFC GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI

    LKKIAATIIS FMQVQKCTIF IVDEDCSDSF SSVFHMECEE LEKSSDTLTR

    EHDANKINYM YAQYVKNTME PLNIPDVSKD KRFPWTTENT GNVNQQCIRS

    LLCTPIKNGK KNKVIGVCQL VNKMEENTGK VKPFNRNDEQ FLEAFVIFCG

    LGIQNTQMYE AVERAMAKQM VTLEVLSYHA SAAEEETREL QSLAAAVVPS

    AQTLKITDFS FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL

    SVKKNYRKNV AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LEILALLIAA

    LSHDLDHRGV NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI

    LSGLSIEEYK TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFNLEDPH

    QKELFLAMLM TACDLSAITK PWPIQQRIAE LVATEFFDQG DRERKELNIE

    PTDLMNREKK NKIPSMQVGF IDAICLQLYE ALTHVSEDCF PLLDGCRKNR

    QKWQALAEQQ EKMLINGESG QAKRN

  • UCSC uc003idf.4; human. [O76074-1];


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