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Catalog
C000133
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Alternative name
Ubiquitin-conjugating enzyme E2 L3 CLIA KIT; L-UBC CLIA KIT; UbcH7 CLIA KIT; Ubiquitin carrier protein L3 CLIA KIT; Ubiquitin-conjugating enzyme E2-F1 CLIA KIT; Ubiquitin-protein ligase L3UBE2L3 CLIA KIT; UBCE7 CLIA KIT; UBCH7 CLIA KIT
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GeneUBE2L3
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2L3 (UBE2L3).
No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) and analogues was observed.
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SamplesTissue homogenates and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 5.28pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) level in the sample or standard.
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