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Human BIN1 ELISA Kit

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  • Alternative name

    Human amphiphysin II ELISA Kit;Human amphiphysin-like protein ELISA Kit;Human box-dependent myc-interacting protein 1 ELISA Kit;Human bridging integrator 1 ELISA Kit;Human AMPHL ELISA Kit;Human AMPH2 ELISA Kit;Human SH3P9 ELISA Kit;Human myc box-dependent-interacting protein 1 ELISA Kit;Human box dependant MYC interacting protein 1 ELISA Kit;
  • Catalog
    E005317
  • species
    Human
  • GeneBIN1
  • Standard CurveHuman BIN1 ELISA Kit
  • Other Species Mouse Bin1 ELISA KitRat Bin1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Bridging Integrator 1 (BIN1). No significant cross-reactivity or interference between Bridging Integrator 1 (BIN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman BIN1 ELISA Kit allows for the in vitro quantitative determination of BIN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bridging Integrator 1 (BIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bridging Integrator 1 (BIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bridging Integrator 1 (BIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bridging Integrator 1 (BIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Myc box-dependent-interacting protein 1 Protein information
  • Uniprot ID BIN1_HUMAN
  • Uniprot AC O75514; O75515; O75516; O75517; O75518; Q659B7; Q92944; Q99688;
  • UniGene Hs.193163;
  • GeneID 274
  • KEGG hsa:274;
  • Human Myc box-dependent-interacting protein 1 Protein SEQUENCE

  • SEQUENCE 593 AA; 64699 MW; 0FF1956F0C7E3B50 CRC64;

    MAEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN

    FNKQLTEGTR LQKDLRTYLA SVKAMHEASK KLNECLQEVY EPDWPGRDEA

    NKIAENNDLL WMDYHQKLVD QALLTMDTYL GQFPDIKSRI AKRGRKLVDY

    DSARHHYESL QTAKKKDEAK IAKPVSLLEK AAPQWCQGKL QAHLVAQTNL

    LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN TFQSIAGLEE

    NFHKEMSKLN QNLNDVLVGL EKQHGSNTFT VKAQPSDNAP AKGNKSPSPP

    DGSPAATPEI RVNHEPEPAG GATPGATLPK SPSQLRKGPP VPPPPKHTPS

    KEVKQEQILS LFEDTFVPEI SVTTPSQFEA PGPFSEQASL LDLDFDPLPP

    VTSPVKAPTP SGQSIPWDLW EPTESPAGSL PSGEPSAAEG TFAVSWPSQT

    AEPGPAQPAE ASEVAGGTQP AAGAQEPGET AASEAASSSL PAVVVETFPA

    TVNGTVEGGS GAGRLDLPPG FMFKVQAQHD YTATDTDELQ LKAGDVVLVI

    PFQNPEEQDE GWLMGVKESD WNQHKELEKC RGVFPENFTE RVP

  • UCSC uc002tns.3; human. [O00499-1];


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