Human AKT1 ELISA Kit

Alternative name
Human Protein kinase B ELISA Kit;Human PKB ELISA Kit;Human protein kinase B alpha ELISA Kit;Human PKB alpha ELISA Kit;Human proto-oncogene c-Akt ELISA Kit;Human RAC-PK-alpha ELISA Kit;Human RAC ELISA Kit;Human AKT ELISA Kit;Human CWS6 ELISA Kit;Human PKB-ALPHA ELISA Kit;Human PRKBA ELISA Kit;Human RAC-ALPHA ELISA Kit;Human AKT serine/threonine kinase 1 ELISA Kit;Human RAC-alpha serine/threonine-protein kinase ELISA Kit;Human AKT1m ELISA Kit;Human rac protein kinase alpha ELISA Kit;Human serine-threonine protein kinase ELISA Kit;Human v-akt murine thymoma viral oncogene homolog 1 ELISA Kit;Human v-akt murine thymoma viral oncogene-like protein 1 ELISA Kit;
Catalog
E032726
species
Human
GeneAKT1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman AKT1 ELISA Kit allows for the in vitro quantitative determination of AKT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PKB alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PKB alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PKB alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PKB alpha can be calculated.
Human RAC-alpha serine/threonine-protein kinase Protein information
Uniprot ID AKT1_HUMAN
Uniprot AC P31749; B2RAM5; B7Z5R1; Q9BWB6;
UniGene Hs.525622;
GeneID 207
KEGG hsa:207;
Human RAC-alpha serine/threonine-protein kinase Protein SEQUENCE
SEQUENCE 480 AA; 55686 MW; 6EAFF4F8AD436714 CRC64;
MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA
PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT
TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF
EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR
VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA
RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY
NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK
EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT
PPDQDDSMEC VDSERRPHFP QFSYSASGTA
UCSC uc001ypk.4; human. [P31749-1];

Category

Human AKT1 ELISA Kit