Human AKT1 ELISA Kit

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  • Alternative name

    Human Protein kinase B ELISA Kit;Human PKB ELISA Kit;Human protein kinase B alpha ELISA Kit;Human PKB alpha ELISA Kit;Human proto-oncogene c-Akt ELISA Kit;Human RAC-PK-alpha ELISA Kit;Human RAC ELISA Kit;Human AKT ELISA Kit;Human CWS6 ELISA Kit;Human PKB-ALPHA ELISA Kit;Human PRKBA ELISA Kit;Human RAC-ALPHA ELISA Kit;Human AKT serine/threonine kinase 1 ELISA Kit;Human RAC-alpha serine/threonine-protein kinase ELISA Kit;Human AKT1m ELISA Kit;Human rac protein kinase alpha ELISA Kit;Human serine-threonine protein kinase ELISA Kit;Human v-akt murine thymoma viral oncogene homolog 1 ELISA Kit;Human v-akt murine thymoma viral oncogene-like protein 1 ELISA Kit;

  • Catalog
    E032726
  • species
    Human
  • GeneAKT1
  • Other Species Mouse Akt1 ELISA KitBovine AKT1 ELISA KitRat Akt1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman AKT1 ELISA Kit allows for the in vitro quantitative determination of AKT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PKB alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PKB alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PKB alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PKB alpha can be calculated.
  • Human RAC-alpha serine/threonine-protein kinase Protein information
  • Uniprot ID AKT1_HUMAN
  • Uniprot AC P31749; B2RAM5; B7Z5R1; Q9BWB6;
  • UniGene Hs.525622;
  • GeneID 207
  • KEGG hsa:207;
  • Human RAC-alpha serine/threonine-protein kinase Protein SEQUENCE
  • SEQUENCE 480 AA; 55686 MW; 6EAFF4F8AD436714 CRC64;

    MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA

    PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT

    TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF

    EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR

    VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA

    RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI

    KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY

    NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK

    EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT

    PPDQDDSMEC VDSERRPHFP QFSYSASGTA

  • UCSC uc001ypk.4; human. [P31749-1];


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