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Alternative name
HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T antibody
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Catalog
A009185
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IF,IP
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PurificationImmunogen affinity purified
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Immunogenubiquitin-conjugating enzyme E2T (putative)
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A009185(UBE2T antibody) at dilution of 1:800
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Recommended dilutionWB : 1:200-1:2000;IP:1:200-1:2000；IF：1:50-1:500
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Product Description specificalThe ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
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Gene nameUBE2T
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locationNucleus
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Uniprot IDQ9NPD8
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Calculated M.W.23kDa
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Observed M.W.23 kDa
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