Human A2ML1 ELISA Kit

Alternative name
Human C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9 ELISA Kit;Human CPAMD9 ELISA Kit;Human p170 ELISA Kit;Human alpha-2-macroglobulin like 1 ELISA Kit;Human alpha-2-macroglobulin-like protein 1 ELISA Kit;Human C3 and PZP-like, alpha-2-macroglobulin domain containing 9 ELISA Kit;
Catalog
E001585
species
Human
GeneA2ML1
Standard Curve
Other Species Mouse A2ML1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin Like Protein 1 (a2ML1). No significant cross-reactivity or interference between Alpha-2-Macroglobulin Like Protein 1 (a2ML1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.27ng/mL.
Intended UseHuman A2ML1 ELISA Kit allows for the in vitro quantitative determination of A2ML1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin Like Protein 1 (a2ML1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin Like Protein 1 (a2ML1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin Like Protein 1 (a2ML1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin Like Protein 1 (a2ML1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-2-macroglobulin-like protein 1 Protein information
Uniprot ID A2ML1_HUMAN
Uniprot AC Q8N1M4; Q96LQ8;
UniGene Hs.620532;
GeneID 144568
KEGG hsa:144568;
Human Alpha-2-macroglobulin-like protein 1 Protein SEQUENCE
SEQUENCE 1454 AA; 161107 MW; 15ED65D000834E33 CRC64;
MWAQLLLGML ALSPAIAEEL PNYLVTLPAR LNFPSVQKVC LDLSPGYSDV
KFTVTLETKD KTQKLLEYSG LKKRHLHCIS FLVPPPAGGT EEVATIRVSG
VGNNISFEEK KKVLIQRQGN GTFVQTDKPL YTPGQQVYFR IVTMDSNFVP
VNDKYSMVEL QDPNSNRIAQ WLEVVPEQGI VDLSFQLAPE AMLGTYTVAV
AEGKTFGTFS VEEYVLPKFK VEVVEPKELS TVQESFLVKI CCRYTYGKPM
LGAVQVSVCQ KANTYWYREV EREQLPDKCR NLSGQTDKTG CFSAPVDMAT
FDLIGYAYSH QINIVATVVE EGTGVEANAT QNIYISPQMG SMTFEDTSNF
YHPNFPFSGK IRVRGHDDSF LKNHLVFLVI YGTNGTFNQT LVTDNNGLAP
FTLETSGWNG TDVSLEGKFQ MEDLVYNPEQ VPRYYQNAYL HLRPFYSTTR
SFLGIHRLNG PLKCGQPQEV LVDYYIDPAD ASPDQEISFS YYLIGKGSLV
MEGQKHLNSK KKGLKASFSL SLTFTSRLAP DPSLVIYAIF PSGGVVADKI
QFSVEMCFDN QVSLGFSPSQ QLPGAEVELQ LQAAPGSLCA LRAVDESVLL
LRPDRELSNR SVYGMFPFWY GHYPYQVAEY DQCPVSGPWD FPQPLIDPMP
QGHSSQRSII WRPSFSEGTD LFSFFRDVGL KILSNAKIKK PVDCSHRSPE
YSTAMGAGGG HPEAFESSTP LHQAEDSQVR QYFPETWLWD LFPIGNSGKE
AVHVTVPDAI TEWKAMSFCT SQSRGFGLSP TVGLTAFKPF FVDLTLPYSV
VRGESFRLTA TIFNYLKDCI RVQTDLAKSH EYQLESWADS QTSSCLCADD
AKTHHWNITA VKLGHINFTI STKILDSNEP CGGQKGFVPQ KGRSDTLIKP
VLVKPEGVLV EKTHSSLLCP KGKVASESVS LELPVDIVPD STKAYVTVLG
DIMGTALQNL DGLVQMPSGC GEQNMVLFAP IIYVLQYLEK AGLLTEEIRS
RAVGFLEIGY QKELMYKHSN GSYSAFGERD GNGNTWLTAF VTKCFGQAQK
FIFIDPKNIQ DALKWMAGNQ LPSGCYANVG NLLHTAMKGG VDDEVSLTAY
VTAALLEMGK DVDDPMVSQG LRCLKNSATS TTNLYTQALL AYIFSLAGEM
DIRNILLKQL DQQAIISGES IYWSQKPTPS SNASPWSEPA AVDVELTAYA
LLAQLTKPSL TQKEIAKATS IVAWLAKQHN AYGGFSSTQD TVVALQALAK
YATTAYMPSE EINLVVKSTE NFQRTFNIQS VNRLVFQQDT LPNVPGMYTL
EASGQGCVYV QTVLRYNILP PTNMKTFSLS VEIGKARCEQ PTSPRSLTLT
IHTSYVGSRS SSNMAIVEVK MLSGFSPMEG TNQLLLQQPL VKKVEFGTDT
LNIYLDELIK NTQTYTFTIS QSVLVTNLKP ATIKVYDYYL PDEQATIQYS
DPCE
UCSC uc001quz.6; human. [A8K2U0-1];

Category

Human A2ML1 ELISA Kit

Human C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9 ELISA Kit;Human CPAMD9 ELISA Kit;Human p170 ELISA Kit;Human alpha-2-macroglobulin like 1 ELISA Kit;Human alpha-2-macroglobulin-like protein 1 ELISA Kit;Human C3 and PZP-like, alpha-2-macroglobulin domain containing 9 ELISA Kit;