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Alternative name
DKFZp686B04100, ISGF 3, STAT1, STAT91 antibody
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Catalog
A008295
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,FC
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PurificationImmunogen affinity purified
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Immunogensignal transducer and activator of transcription 1, 91kDa
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IHCImmunohistochemistry of paraffin-embedded human breast cancer tissue slide using A008295( STAT1 Antibody) at dilution of 1:50
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Western BlotPC-3 cells were subjected to SDS PAGE followed by western blot with A008295(STAT1 antibody) at dilution of 1:600
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalSignal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
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Gene nameSTAT1
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Uniprot IDP42224
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Observed M.W.84 kDa
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