StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman,Mouse ,Rat
PurificationImmunogen affinity purified
Immunogensplicing factor 3b, subunit 4, 49kDa
IHCImmunohistochemistry of paraffin-embedded human pancreas cancer using A007780(SF3B4 antibody) at dilution of 1:50
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A007780(SF3B4 antibody) at dilution of 1:500
Recommended dilutionWB : 1:200-1:2000
Product Description specificalSubunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12- dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.